Quality Control and Assurance in Cell Culture
Expert-defined terms from the Certified Specialist Programme in Cell Culture Optimization course at Greenwich School of Business and Finance. Free to read, free to share, paired with a professional course.
Aseptic Technique refers to a set of practices used to minimize the presence of… #
Related terms include Antimicrobial Agents, Contamination Control, and Sterilization. Aseptic technique is essential in cell culture to prevent contamination and ensure the health and integrity of cells. This involves using filtered pipette tips, sterile culture vessels, and working in a laminar flow hood.
Bioassay is a quantitative measure of the effect of a substance on a cell… #
Related terms include Cell Viability Assay, Cytotoxicity Assay, and Dose-Response Curve. Bioassays can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Cell Banking refers to the process of preserving and storing cell culture… #
Related terms include Cell Line Authentication, Cell Line Characterization, and Cell Storage. Cell banking is critical for maintaining the integrity and consistency of cell cultures, and involves the use of controlled rate freezers and liquid nitrogen storage.
Cell Culture Medium is a nutrient #
rich solution used to support the growth and survival of cells in culture, often containing amino acids, carbohydrates, and salts. Related terms include Cell Culture Supplements, Fetal Bovine Serum, and Serum-Free Medium. Cell culture medium can be customized to meet the specific needs of a cell type, and may include growth factors, hormones, and antibiotics.
Cell Line Authentication is the process of verifying the identity and … #
Related terms include Cell Line Characterization, Cell Line Contamination, and Cell Line Misidentification. Cell line authentication is essential for maintaining the integrity of cell cultures, and involves the use of molecular biological techniques such as PCR and DNA sequencing.
Cell Line Characterization is the process of evaluating the biological an… #
Related terms include Cell Line Authentication, Cell Line Validation, and Cell Line Verification. Cell line characterization is critical for understanding the biology of a cell line, and involves the use of biochemical and biophysical techniques such as flow cytometry and microscopy.
Cell Proliferation is the process by which cells grow and divide t… #
Related terms include Cell Growth, Cell Division, and Cell Survival. Cell proliferation is essential for maintaining the health and viability of cell cultures, and can be influenced by growth factors, hormones, and nutrients.
Cell Viability Assay is a quantitative measure of the number of living… #
Related terms include Bioassay, Cytotoxicity Assay, and Cell Proliferation Assay. Cell viability assays can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Contamination Control refers to the practices used to minimize the presence of <… #
Related terms include Aseptic Technique, Antimicrobial Agents, and Sterilization. Contamination control is essential for maintaining the health and integrity of cell cultures, and involves the use of filtered pipette tips, sterile culture vessels, and working in a laminar flow hood.
Cryopreservation is the process of preserving cells or tissues at very <b… #
Related terms include Cell Banking, Cell Storage, and Freeze-Thaw Cycle. Cryopreservation is critical for maintaining the integrity and consistency of cell cultures, and involves the use of controlled rate freezers and cryoprotectants.
Cytotoxicity Assay is a quantitative measure of the toxic effect o… #
Related terms include Bioassay, Cell Viability Assay, and Dose-Response Curve. Cytotoxicity assays can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Dose #
Response Curve is a graphical representation of the relationship between the dose of a substance and its effect on a cell culture, often used to evaluate the potency and efficacy of a treatment. Related terms include Bioassay, Cell Viability Assay, and Cytotoxicity Assay. Dose-response curves can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Fetal Bovine Serum is a nutrient #
rich supplement used to support the growth and survival of cells in culture, often containing growth factors, hormones, and proteins. Related terms include Cell Culture Medium, Cell Culture Supplements, and Serum-Free Medium. Fetal bovine serum is commonly used in cell culture, but can be variable in composition and may contain contaminants.
Freeze #
Thaw Cycle refers to the process of freezing and thawing cells or tissues, often used to evaluate the viability and integrity of cells after cryopreservation. Related terms include Cell Banking, Cell Storage, and Cryopreservation. Freeze-thaw cycles can be used to evaluate the response of cells to cryopreservation and thawing, and may involve the use of cryoprotectants to minimize damage.
Growth Factor is a protein or peptide that stimulates cell … #
Related terms include Cell Culture Medium, Cell Culture Supplements, and Hormone. Growth factors can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Hormone is a chemical messenger that regulates cell beha… #
Related terms include Cell Culture Medium, Cell Culture Supplements, and Growth Factor. Hormones can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Immortalization is the process by which cells become capable of indefi… #
Related terms include Cell Line Authentication, Cell Line Characterization, and Cell Line Validation. Immortalization can be achieved through the use of oncogenes, telomerase, or other genetic elements.
Laminar Flow Hood is a controlled environment used to minimize the presen… #
Related terms include Aseptic Technique, Contamination Control, and Sterilization. Laminar flow hoods are essential for maintaining the health and integrity of cell cultures, and involve the use of HEPA filters and sterile equipment.
Microscopy is a technique used to visualize and analyze cells and… #
Related terms include Cell Line Characterization, Cell Morphology, and Cell Behavior. Microscopy can be used to evaluate the morphology and behavior of cells, and may involve the use of stains or markers to visualize specific structures or proteins.
Myccoplasma Detection is the process of identifying and eliminating</b… #
Related terms include Cell Line Authentication, Cell Line Characterization, and Cell Line Contamination. Mycoplasma detection is essential for maintaining the health and integrity of cell cultures, and involves the use of specific primers or antibodies to detect mycoplasma DNA or proteins.
Passaging is the process of transferring cells from one culture vessel to… #
Related terms include Cell Culture, Cell Proliferation, and Cell Survival. Passaging can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Quality Control refers to the practices used to ensure the quality and <b… #
Quality control is essential for maintaining the integrity and reliability of cell cultures, and involves the use of quality control checks and validation procedures.
Serum #
Free Medium is a nutrient-rich solution used to support the growth and survival of cells in culture, often containing growth factors, hormones, and proteins. Related terms include Cell Culture Medium, Cell Culture Supplements, and Fetal Bovine Serum. Serum-free medium is commonly used in cell culture, and can be customized to meet the specific needs of a cell type.
Sterilization is the process of eliminating all microorganisms fro… #
Related terms include Aseptic Technique, Contamination Control, and Laminar Flow Hood. Sterilization is essential for maintaining the health and integrity of cell cultures, and involves the use of autoclaves, ovens, or chemical sterilants.
Tissue Culture refers to the practice of growing and maintaining c… #
Tissue culture is used to study the biology of cells and tissues, and involves the use of standardized protocols and validation procedures.
Validation refers to the process of verifying the accuracy and … #
Related terms include Cell Line Authentication, Cell Line Characterization, and Quality Control. Validation is essential for maintaining the integrity and reliability of cell cultures, and involves the use of validation procedures and quality control checks.
Viability Assay is a quantitative measure of the number of living … #
Viability assays can be used to evaluate the response of cells to various stimuli, including drugs, hormones, and growth factors.
Xenobiotic is a foreign substance that is not naturally found in a… #
Xenobiotics can be used to evaluate the metabolism and toxicity of substances in cell culture, and may involve the use of specific enzymes or transporters.